Chirality is a key factor in the efficacy of many drug products, and thus the production of single enantiomers of chiral intermediates has become increasingly important in the pharmaceutical industry. Single enantiomers can be produced by chemical or chemo-enzymatic synthesis. The advantages of biocatalysis over chemical synthesis are that enzyme-catalyzed reactions are often highly enantioselective and regioselective. They can be carried out at ambient temperature and atmospheric pressure, thus avoiding the use of more extreme conditions which could cause problems with isomerization, racemization, epimerization, and rearrangement. Microbial cells and enzymes derived there from can be immobilized and reused for many cycles. In addition, enzymes can be overexpressed to make biocatalyst processes economically efficient, and enzymes with modified activity can be tailor-made. This presentation describe the biocatalytic preparation of chiral intermediates for the synthesis drugs.