Wednesday, July 29, 2009 - 8:30 AM
S98
Bioethanol production process from woody biomass using fungal and microwave irradiation pretreatments and genetically engineered bacteria
Takashi Watanabe, Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan and Hideshi Yanase, Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyamacho-minami, Tottori, 680-8552, Japan.
There is growing demand to develop new, highly efficient technology to produce bioethanol from woody biomass. In enzymatic conversion of lignocellulosics it is necessary to decompose the network of lignin prior to the enzymatic hydrolysis because lignin makes the access of cellulolytic and hemicellulolytic enzymes to the cell wall polysaccharides difficult. Efficient conversion of the enzymatic hydrolyzates by ethanologenic microbes are also the key factor for bioethanol production. In our NEDO project (R&D member: RISH, Kyoto Univ., Fac. Eng. Tottori Univ., Japan Chemical Engineering & Machinery Co. Ltd., and Toyota Motor Corporation), we have applied ligninolytic systems of selective white rot fungi and microwave-assisted solvolysis to the pretreatment. We have developed new continuous microwave reactors by applying 3D electromagnetic simulation technique. Through cell-surface engineering based on genome DNA analysis information, novel, high-performance ethanologenic bacteria, Zymobacter palmae and Zymomonas mobilis, which efficiently convert hexoses and pentoses to bioethanol, secrete β-glucosidase, and display cellulase on the surface of the cells, are being bred. A high-performance fermentation process using the ethanologenic bacteria is being developed to produce bioethanol from Japanese cedar and other woody biomass. A bench scale plant for bioethanol production using the pretreatment system and genetically engineered bacteria is planned to build in 2010.
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See more of The Annual Meeting and Exhibition 2009 (July 26 - 30, 2009)