Wednesday, July 29, 2009 - 10:30 AM
S101
Development of environmentally benign and consolidated process for efficient production of cellulosic ethanol
Akihiko Kondo, Tomohisa Hasunuma, Tsutomu Tanaka, and Chiaki Ogino. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan
We are trying to develop a consolidated bioprocess (CBP) for highly efficient ethanol production from cellulosic biomass. The process is consisting of 1) biomass preprocessing process that consists of partial decomposition and enzyme treatment after hydrothermal treatment, 2) simultaneous saccharification and ethanol fermentation process by using super microbial cells, 3) an energy-saving ethanol-concentration /dehydration process by using advanced HIDiC (Heat Integrated Distillation Column).
For the reduction of the cost of bioethanol production, the development of super- microbial cells for CBP is very important. Among ethanol-producing microbial strains, yeast Saccharomyces cerevisiae has several advantages owing to its high ethanol production from hexoses and high tolerance to ethanol and other inhibitory compounds in the hydrolysates of lignocellulosic biomass. The cell surface engineering is one of the key technologies for the development of yeast strain for CBP. We have developed CBP yeast strains that display both cellulase and hemicellulase on the surface of the cells in which the metabolic pathway is modified for ethanol production from both C5 and C6 sugars. The displayed enzymes are regarded as a kind of self-immobilized enzyme on the cell surfaces. Due to the display of these enzymes, cellulosic materials were sequentially hydrolyzed to C5 and C6 sugars on the yeast cell surface, immediately utilized and converted to ethanol by intracellular enzymes. In this CBP yeast strain, the yield in terms of grams of ethanol produced per grams of carbohydrate utilized was found to be high
For the reduction of the cost of bioethanol production, the development of super- microbial cells for CBP is very important. Among ethanol-producing microbial strains, yeast Saccharomyces cerevisiae has several advantages owing to its high ethanol production from hexoses and high tolerance to ethanol and other inhibitory compounds in the hydrolysates of lignocellulosic biomass. The cell surface engineering is one of the key technologies for the development of yeast strain for CBP. We have developed CBP yeast strains that display both cellulase and hemicellulase on the surface of the cells in which the metabolic pathway is modified for ethanol production from both C5 and C6 sugars. The displayed enzymes are regarded as a kind of self-immobilized enzyme on the cell surfaces. Due to the display of these enzymes, cellulosic materials were sequentially hydrolyzed to C5 and C6 sugars on the yeast cell surface, immediately utilized and converted to ethanol by intracellular enzymes. In this CBP yeast strain, the yield in terms of grams of ethanol produced per grams of carbohydrate utilized was found to be high
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See more of Invited Oral Papers
See more of The Annual Meeting and Exhibition 2009 (July 26 - 30, 2009)