Sunday, July 26, 2009
P93
Detection and Characterization of Water-borne Norovirus in Gyeonggi Province Area, South Korea
Noroviruses have been emerged as a leading cause of outbreak of acute viral gastroenteritis. Norovirus infections often result from ingesting contaminated water and food.
Objective:
1. Investigation and characterization of water-borne norovirus genotypes detected in Gyeonggi Province area, South Korea
2. Epidemiological comparison of norovirus genotypes between clinical and water samples.
Methods:
A total of 50 various water samples were filtered, eluted and concentrated. Water-borne noroviruses was detected by RT-PCR and real-time RT-PCR. Norovirus genotypes were determined by sequencing and phylogenetic analysis.
Results:
1. Twelve samples (24% of the total) turned out to be norovirus positive by semi-nested RT-PCR. 2. We also adopted real-time RT-PCR and newly developed 3´-minor groove binder (MGB)-DNA probe, and then validated with norovirus-positive human stool samples. Noroviruses were detected in 10 water samples (20% of the total) by real-time RT-PCR. 3. Seven different genotypes (Genogroup I-4, -5, -9, -10, and Genogroup II-3, -4, -7) were identified in water samples. Furthermore, norovirus genotypes detected in water samples were mostly coincided with genotype data of human clinical samples from Gyeonggi Province area.
Conclusions:
1. Water-borne noroviruses circulating in Gyeonggi Province area have been identified from source water, stream water and ground water samples by RT-PCR and real-time RT-PCR.
2. Molecular epidemiological information showed that genotypes of water-borne norovirus were also detected from human clinical samples collected around the same area.
References:
Kageyama T, Kojima S, Shinohara M, Uchida K, Fukushi S, Hoshino FB, Takeda N, Katayama K., Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J Clin Microbiol. (2003), 41. 1548-57
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