Monday, July 27, 2009
P64

Effect of various inhibitory compounds in lignocellulosic hydrolysates on the butanol production and the growth of solventogenic Clostridia

Min-Ok Cho1, Sun-Mi Lee2, Youngsoon Um1, Dae Haeng Cho3, Yong Hwan Kim3, and Byoung-In Sang1. (1) Center for Environmental Technology Research, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul, South Korea, (2) Hazardous substance research center, Korea Institute of Science and Technology, 131, Cheongryang, Seoul, South Korea, (3) Dept. of Chemical engineering, Kwangwoon University, 447-1, Wolgye-Dong, Nowon-Gu, Seoul, South Korea

Biobutanol has many superior properties as an alternative fuel when compared to ethanol. Fermentative substrate is an important factor influencing the cost of butanol production. Lignocelluloses and hemicelluloses are the most abundant renewable resource on the planet, and have attracted much attention recently due to their potential as substrates for biofuel production. The lignocellulosic hydolysate, however, contain not only fermentable sugars but also non-fermentable compounds such as weak acids and various phenolic compounds that inhibit microbial fermentation. In this study, we investigated the effect of various inhibitory phenolic compounds, ferulic acid, syringaldehyde, ρ-coumaric acid, 4-hydroxybenzoic acid, vanilin acid, and vanilin, on the butanol production and the growth of solventogenic Clostridia, Clostridium beijerinckii NCIMB 8052 and Clostridium acetobutylicum ATCC 824. The effect of the inhibitory compounds on the bacterial growth and the butanol production were monitored over 72-hour of batch cultivation with C. beijerinckii NCIMB 8052 and C. acetobutylicum ATCC 824. The exposure to ρ-coumaric acid led a reduced butanol production for both C. beijerinckii NCIMB 8052 and C. acetobutylicum ATCC 824, while the addition of syringaldehyde did not show any reduction on butanol production by both strains.

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