Sunday, July 26, 2009
P87

Biosurfactant-producing Indigenous bacteria for Enhancing Oil Recovery in a Petroleum Reservoir after Polymer Flooding

Yuehui She1, Fan Zhang2, Zhengliang Wang2, Jinfeng Huang3, Longjiang Yu1, and Wenjun Wang3. (1) School of Life Science and Technology, Huazhong University of Science and Technology, No.1037Luoyu Road, Wuhan, 430074, China, (2) College of Chemical and Environmental Engineering, Yangtze University, No.1, Nanhu, Jingzhou, 434023, China, (3) The third Oil Production Plant of Daqing Oilfield Co. Ltd., Daqing, Ranghu Road, Daqing, 163113, China

Abstract: Biosurfactant-producing indigenous bacteria (BPIB) are isolated from the petroleum reservoir after polymer flooding in Daqing Oilfield (DQPRPF, China). The experiment results indicate that three BPIB, named XDS1, XDS2 and XDS3, isolated and screened from the produced fluid, are identified as bacillus with following properties, short rods, white color, round shape, protrude structure and rough surface. Strains have polar flagella, are able to produce endospores and are sporangia definitely swollen, terminal. The surface property experiment shows that the diameter of oil spreading circle of the fermentation broth are more than 4.5cm, with about 30mN/m surface tension by using liquid wax of 1% as the carbon source at 45°C with 167rpm/min. Three strains grow well and reach their maximum growth after they are cultured for six hours. A lot of oil releasing agents can be produced by continuously adding nutriments to activate the three BPIB and degrade petroleum hydrocarbon. The oil degradation rates of three screened BPIB in DQPRPF are all over 50%, and the maximum degradation ratio is reached 84%. At the same time, the heavy components of crude oil are changed into light components and the mobility property of crude oil is improved too. The surface tension between culture broth and air was decreased to about 30 mN/m, and the viscosity of crude oil have decreased by about 20% after three strains have effects on the crude oil for three days. It is recommended that these three BPIB to be applied in DQPRPF to enhance oil recovery.

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