Sunday, July 26, 2009
P81
Effect of culture conditions on the production of an extracellular protease by a Bacillus sp. isolated from soil sample of Lavizan Jungle Park
Soil samples of Tehran jungle parks included Lavizan,Chitgar, Sorhke hesar, Taleghani and Khojr were screened for proteolytic Bacilli. Among eighteen protease producers one of the isolates obtained from Lavizan park , in north east of Tehran , was selected for further experimental studies. This isolate was identified as Bacillus.sp based cellular morphlogy, gram staining, biochemical tests and partial sequence of 16S rRNA .Various nutritional and environmental parameters affected protease production by Bacillus.sp.Protease production by Bacillus.sp cultivated in liquid cultures containing 1% starch as a carbon source and 0.4% corn steep liquor as a nitrogen source reached a maximum at 24 h, with levels of 340.908 U/mL. Starch and maltose were the best substrates for enzyme production while some pure sugars such as fructose, glucose and sucrose could not influence production of protease. Among various organic nitrogen sources corn steep liquor, which is commercial , was found as a best substrate followed by yeast extract, whey protein and beef extract. Bacillus.sp Could not utilize urea as an inorganic nitrogen source to grow and produce protease.The optimal pH and optimal temperature of enzyme production were 8.0 and 45°C , respectively. Studies on enzymatic characterization revealed that crude protease showed maximum activity at pH 9.0 and 60°C, which it is indicating the enzyme to be thermo-alkaline protease .
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