Monday, July 27, 2009
P116
Cost- effective production and optimization of alkaline-active xylanase, by an indigenous strain of Bacillus mojavensis isolated from cotton farm
Shokoofeh Ghazi and Abbas akhavan Sepahi. Microbiology, Islamic azad university, Darrband ST,Tajrish squer, Tehran, 98, Iran
An indigenous strain of Bacillus mojavensis was isolated from soil of cotton farm (Kashan-Iran). It was shown to produce alkaline-active and stable xylanase enzyme. The highest activity of enzyme was obtained on phosphate buffer at pH 9.0, 55 oC. Different agricultural residuals were evaluated as a substrate in submerged fermentation for xylanase production by this strain. Appropriate level of xylanase production equal to 249.308 IU/ml achieved at pH 8 and 37 oC, within 48 h fermentation in enzyme basic production medium supplemented with oat bran as an optimum carbon source and yeast extract plus tryptone as optimum nitrogen sources. Agitation rate, 200 rpm and inoculum size 2% v/v, found to be optimal for maximum enzyme production. Finally, xylanase production from 194.68 IU/ml under non-optimized fermentation elevated to 302.466 IU/ml in optimized condition. The produced xylanase, presented 75% and 74% stability at pH 9 and 10 for 2 h. Also at 60 oC , 70% and at 55o and 50 oC, 56% and 50% residual enzyme activity were measured during 30 min . Therefore, produced xylanase was introduced as an alkaline-active and stable xylanase displaying suitable thermostability feature. Considering to the high production level, optimum enzyme activity and enzyme stability features, these reveal the superior character of bacillus mojavensis as a suitable candidate for industrial-suited xylanase production.
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