Sunday, July 26, 2009
P131
Metabolic engineering of E. coli for farnesol production
Terpenoids are a large and diverse class of naturally-occurring compounds. They are present in all living organisms and include many important drugs, valuable flavor and fragrance compounds, pigments, antioxidants, steroids and natural polymers. They are derived from five-carbon universal building blocks (IPP, DMAPP)assembled and modified in various ways. The increased synthesis of building blocks of IPP and DMAPP through metabolic engineering is a way to enhance the production of terpenoids. IPP and DMAPP supply can be increased significantly through the introduction of foreign MVA (mevalonate) pathway. Farnesol is an acyclic sesquiterpene alcohol present in many essential oils of plants. It is used in perfumery to emphasize the odors of sweet floral perfumes and is also applied to a natural pesticide for mites and a pheromone for several other insects. Farnesol can be formed by depyrophosphorylation of FPP (farnesyl diphosphate). FPP is synthesized by FPP synthase (IspA) condensing two IPP and one DMAPP. The recombinant E. coli harboring foreign MVA pathway produced approximately 70mg/L of farnesol by overexpression of IspA. The culture was carried out with dodecane overlay over culture broth because of hydrophobic and volatile properties of farnesol. The high amount of farnesol production was previously reported to 28mg/L in the metabolic engineered yeast. Therefore, this work demonstrates the feasibility of farnesol production using E. coli as a host and its metabolic engineering. This work was supported by the 21C Frontier Microbial Genomics and Applications Center Program, EB-NCRC (Grant No. R15-2003-012-02001-0), and BK21 program of Korea.
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