Monday, July 27, 2009
P104

Studies on Biosurfactant Production by Pseudomonas aeruginosa PTCC 1561 

Effat Behravan1, Gholamreza Dehghan - Noudeh1, Mohammad Hasan Moshafi2, and Mahsa Sodagar2. (1) Pharmaceutics Reserch Center, Kerman University Of Medical Sciences, -, -, Kerman, -, Iran, (2) Pharmaceutics Research Center, Pharmaceutics Research Center, Kerman University of Medical Sciences,, -, -, Kerman, -, Iran

Biosurfactants are amphipathic molecules which reduce surface and interfacial tensions and synthesized by microorganisms and are widely used in pharmaceutical, cosmetic, food and petroleum industries. Several advantages for biosurfactants in contrast with chemical surfactants, such as lower toxicity; higher biodegradability; better environmental compatibility; higher foaming; high selectivity and specific activity at extreme temperatures, pH, and the ability to be synthesized from renewable feedstock.

In the present study, the production of biosurfactant by Pseudomonas aeruginosa PTCC 1561 has been studied. Pseudomonas aeruginosa was grown in the nutrient broth medium and biosurfactant production was evaluated every 24 h by surface tension and emulsification index (E24). Then P. aeruginosa PTCC 1561 was grown with different conditions in order to get maximum production of biosurfactant. The best culture medium was found to be brain heart infusion broth medium supplemented with starch, Fe3+, Zn2+ and olive oil. After growing the bacteria, the microbial biomass was removed from the supernatant. The amphipathic structure of biosurfactant was established by biochemical and spectroscopy methods and it was confirmed sugar – lipid structure.

Key words: Pseudomonas aeruginosa, bioemulsifer, surface tension, emulsification index


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