Sunday, July 26, 2009
P121
Altered metabolism of reducing power in phosphoglucose isomerase (pgi) mutant Escherichia coli
Susie Kim, Yeong Deok Kwon, and Pil Kim. Dept. of Biotechnology, Catholic Univ. of Korea, Yeokgok 2-dong, Wonmi-gu, Bucheon, South Korea
Glucose-6-phosphate is an intermediate metabolite of glycolysis and a startup precursor of pentose phosphate pathway. To modify the intracellular reducing power metabolism, a mutant Escherichia coli (KS002) was constructed on phosphoglucose isomerase (pgi) which mediating conversion of glucose-6-phosphate into fructose-6-phosphate in the glycolysis. Intracellular NADPH and NADH of KS002 were 53% and 21% increased than those of wild type, respectively, with 48% decrease of growth rate in the minimal glucose medium under aerobic condition. The cell yield (cell mass per glucose consumption) of KS002 and wild type were not very different. The KS002 strain, however, was not able to grow under anaerobic condition using glucose as a sole carbon source. The cell yield of KS002 expressing NADP-dependent malic enzyme (KS002/maeB) was 7.60 g-cell/g-glucose, which is 98% higher yield than that of wild type expressing maeB (4.74 g-cell/g-glucose). The altered metabolism of reducing power, cell yield, and metabolite profile of pgi disrupted E. coli are discussed based on the above results.
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