Tuesday, July 28, 2009 - 9:00 AM
S54

Engineering biocatalysts for industrial succinic acid production

Tammy B. Grabar, Jesus Zaldivar, Xiaohui Yu, James Reinhardt, and Theron Hermann. BioEnergy International, LLC, 42 Cummings Park, Woburn, MA 01801

Volatile petroleum costs and a growing trend towards green chemistry have opened the door to the generation of high volume specialty chemicals by microbial fermentation.  The versatility of succinic acid as an intermediate for chemical conversion to a wide variety of compounds well known in the industry makes succinic acid a very attractive target compound for production from renewable feedstocks.  Myriant Technology LLC (formerly BioEnergy International, LLC) has significantly reduced the costs of producing succinic acid by fermentation.  Utilizing an E. coli bacteria licensed from the University of Florida and developed in the laboratory of Prof. Ingram (Jantama K, Haupt MJ, Svoronos SA, Zhang X, Moore JC, Shanmugam KT, Ingram LO. 2008. Biotechnol. Bioeng. 99:1140-1153), fermentation procedures have been developed to grow the engineered strain to produce high yields of succinate using inexpensive media with short cycle times.