Monday, July 27, 2009
P94
Rosmarious officinalis ethanolic extract protects human lymphocytes against oxidative damage
Javad Behravan, Fatemeh Mosaffa, Gholamreza Karimi, Mehrdad Iranshahi, and Samira SehatBakhsh. Biotechnology Research Center, Mashhad University of Medical Sciences, Vakil abad Boulevard, Mashhad, Iran
The protective properties of Rosmarinus officinalis on the human lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy human volunteers. DNA breaks and resistance to H2O2-induced damage were measured with the comet assay. Human lymphocytes were incubated with six concentrations (10, 25, 50, 100, 200 and 400 μM) of R. officinalis ethanolic extract alone (ROE), or with a combination of H2O2 (25 μM) with ROE (10, 25, 50, 100, 200 and 400 μM) . Ascorbic acid (25, 50, 100, 200 and 400 μM) in a combination with H2O2 25 μM was used as a standard antioxidant agent. Single cells were analyzed with "TriTek Cometscore version 1.5" software. The DNA damage was expressed as % Tail DNA. According to the data, a concentration-dependent increase in protection against DNA damage (from 55.12 to 4.03 %) was observed with ROE concentrations. The observed protective effects may be due to the presense of polyphenol compounds such as carnosic acid, carnosol, rosmarinic acid and other active compounds.
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