Sunday, July 26, 2009
P17
Expression of Trichoderma. reesei enzymes for lignocellulose degradation in homologous and heterologous hosts
Goutami Banerjee, John Scott-Craig, Melissa S. Borrusch, and Jonathan D. Walton. Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824
Enzymes able to release fermentable sugars from biomass feedstocks are one of the major costs in cellulosic ethanol production. Almost all enzyme preparations currently in use are made from genetically modified strains of the filamentous fungus Trichoderma reesei, but the enzymatic activities and relative proportions of these enzyme mixtures are poorly defined. The long-term goal of this project is to produce a less expensive enzyme mixture that is optimized for Ammonia Fiber Explosion (AFEX)-treated corn stover. The first step is to define a ‘minimal enzyme set’ that is capable of releasing Glucose and Xylose from corn stover. A second phase will involve the addition of ‘accessory’ proteins from Trichoderma and other sources to the ‘minimal enzyme set’ to search for possible additive and synergistic effects. The enzymes used for this project are being expressed either in a heterologous host, Pichia pastoris, or in a homologous host, Trichoderma itself. Several expression vectors are being used, with both strong constitutive promoters and strong cellulose-inducible promoters from Trichoderma. It is expected that the optimized mixture will have a higher specific activity than currently available commercial enzyme mixtures.
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See more of The Annual Meeting and Exhibition 2009 (July 26 - 30, 2009)
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See more of The Annual Meeting and Exhibition 2009 (July 26 - 30, 2009)