Monday, July 27, 2009
P90
Bioproduction of Antifungal Compounds for Control of Plant Pathogens by Chromobacterium sp. Strain C61
Myung Ryeol Park1, Ho Seong Choi2, Young Cheol Kim2, Sun Woo Lee3, and In Seon Kim1. (1) Division of Applied Bioscience and Biotechnology, Chonnam National University, Yongbongdong 300, Bukgu, Gwangju, South Korea, (2) Division of Plant Biotechnology, Chonnam National University, Yongbongdong 300, Bukgu, Gwangju, South Korea, (3) Department of Chemistry, Chonnam National University, Yongbongdong 300, Bukgu, Gwangju, South Korea
Chromobacterium sp. strain C61 (C61) exhibits antifungal activity against Rhizoctonia solani which causes damping off disease in rice. In order to identify antifungal compounds produced by C61, C61 cultures were extracted with organic solvents, and the organic extracts were subject to solid phase extraction with silica sorbents. The active eluates were stored at 4°C to obtain precipitates followed by spectroscopy analyses. Two active compounds were identified by MALDI-TOF MS and LC-ESI-MS spectrometer analyses, detecting (M+H)+ peak at m/z 1167 and 1195, respectively. Metabolites showed almost complete inhibition of the growth of R. solani at 100 μg/mL in vitro. This study suggests that C61 is a potential as microbial fungicide against damping off disease, as it produces antifungal metabolites.
Web Page: altair.chonnam.ac.kr/~epsl
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