Sunday, July 26, 2009
P51
Characterization of Escherichia coli Isolates from Poultry and Human Sources by Automated Ribotyping
Cynthia L. Sheffield, Food and Feed Safety, USDA-ARS-Southern Plains Agricultural Center, 2881 F&B Road, College Station, TX 77845, Cecilia R. Cortés, Departamento de Producción Animal: Aves, Facultad de Medicina Veterinaria y Zootecnia;Universidad Nacional Autónoma de México, Circuito Exterior cp 04510 Ciudad Universitaria, Mexico City, Mexico, and Kenneth M. Bischoff, National Center for Agricultural Utilization Research, USDA - Agricultural Research Service, 1815 N. University St., Peoria, IL 61604.
Eighty-nine Escherichia coli isolates from the archival collection of the Universidad Nacional Autónoma de México were analyzed to evaluate the genetic relatedness. E. coli colonies were cultured on Tryptic Soy Agar. Ribotypes were characterized on the RiboPrinter® Microbial Characterization System following DNA digestion with the restriction enzyme EcoRI. A total of 36 ribogroups were isolated. These segregated into three clusters, one cluster contained only poultry isolates, the second contained only human isolates and the third contained 57% poultry isolates and 43% human isolates. Results showed a high level of ribotypic relatedness within the E. coli isolates that could infect both poultry and human sources. While E. coli isolates collected from exclusively poultry or human sources showed a high level of ribotypic diversity.
See more of Poster Session 1
See more of Posters
See more of The Annual Meeting and Exhibition 2009 (July 26 - 30, 2009)
See more of Posters
See more of The Annual Meeting and Exhibition 2009 (July 26 - 30, 2009)