Wednesday, July 29, 2009 - 10:30 AM
S121

Infectious bioagents on environmental surfaces: Standardized testing of liquid chemicals and wiping for decontamination

Syed A. Sattar, Safaa Sabbah, and Susan Springthorpe. Centre for Research on Environmental Microbiology (CREM), University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada

While disinfectants can reduce the risk of spread of bioagents, the testing of such formulations often does not reflect field conditions; nor are they tested with wiping. We used a stringent quantitative carrier test (QCT) to assess selected oxidizers against a microbial cocktail (Acinetobacter baumannii, Mycobacterium terrae, hepatitis A virus, Geobacillus stearothermophilus spores) as surrogates of bioagents. Each disk (1 cm dia.) of brushed stainless steel received 10 μL of the cocktail in a soil load, the inoculum dried and either exposed (5-20 min.) to 50 µL of test disinfectant or wiped (10 sec.; 50-100 g/cm2) with a pre-soaked fabric and the wipe touched to a clean disk for microbial transfer. The disks were eluted and eluates assayed. Peracetic acid (PAA; 1,000 ppm) and accelerated H2O2 (AHP)-based formulations (40,000 to 70,000 ppm) inactivated the spores to undetectable levels, but without complete inactivation of the virus at the same contact time and temperature. Chlorine dioxide (CD) and 5.2% sodium hypochlorite as domestic bleach (DB) showed the broadest spectrum of activity depending on air temperature. While CD (500 and 1,000 ppm) and DB (5,000 ppm) completely inactivated HAV and the spores at room temperature (~24ºC), they only killed the virus at 4ºC but not the spores. M. terrae and A. baumannii were relatively readily inactivated in all tests. In general, the highest level of decontamination and the lowest risk of transfer were achieved when an applied disinfectant was allowed to dry and the surface was subsequently wiped with a disinfectant-soaked fabric.