Monday, July 27, 2009
P78

Screening three strains of Pseudomonas aeruginosa (PTCC 1074, PTCC 1310, PTCC 1430): prediction of biosurfactant – producer strain

Gholamreza Dehghan - Noudeh1, Mohammad Hasan Moshafi2, and Effat Behravan1. (1) Pharmaceutics Reserch Center, Kerman University Of Medical Sciences, -, -, Kerman, -, Iran, (2) Pharmaceutics Research Center, Kerman University of Medical Sciences, -, -, Kerman, -, Iran

Surfactants are amphipathic molecules which widely used in pharmaceutical, cosmetic, food and petroleum industries. Biosurfactant are the structurally diverse group of surface-active molecules synthesize by microorganisms. There are some advantages over the chemical surfactant, such as lower toxicity and higher biodegradability. In the present study, the production of a kind of biosurfactant by three strains of Pseudomonas aeruginosa was investigated. The hemolytic and foam forming activity of different strains were studied and consequently, P. aeruginosa PTCC 1074 was selected as the suitable strain. P. aeruginosa PTCC 1074 was grown in the nutrient broth medium and biosurfactant production was evaluated every 24 h by emulsufucation index and surface tension for the best of production time. After that, in order to get maximum production of biosurfactant, the selected strain was grown with different additives in nutrient broth and the best culture medium was found. The biosurfactant was isolated from the supernatant and its amphipathic structure was confirmed by chemical methods.
Key words: Pseudomonas aeruginosa, biosurfactant, surface tention, emulsification index.

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