Sugarcane processing generates a large volume of ligno-cellulosic materials such as sugarcane leaf and bagasse. Disposal of these agricultural residues is critical for both agricultural profitability and environmental protection. In this study, we demonstrate that cane processed bagasse could be used to produce fuel grade ethanol without enzymatic saccharification. A chemical pre-treatment process using weak acid hydrolysis was applied to remove lignin, which acts as physical barrier to cellulolytic enzymes. Yeast Saccharomyces cerevisiae ATCC strain 765 was used in the experiment. The pre-treatment process effectively removed lignin. Ethanol production in the culture sample was monitored. The results indicate that the highest ethanol production was observed from bagasse treated with 1.4 M H2SO4. The concentration above 1.4 M did not yield higher ethanol yield. Ethanol yield decreased with sulfuric acid concentration above 1.4 M due to the production of inhibitory compounds such as furfural and furfuryl alcochol. This preliminary study showed that ethanol production from post-harvest sugarcane residue such as bagasse is possible without the addition of cellulase enzyme. The ethanol yield in our study is eight times lower than the theoretical yield as per National Renewable Energy Laboratory (NREL) calculation. In this study, we achieved a significant removal of lignin from the bagasse, which resulted in higher production of ethanol. Further research is needed to optimize the conditions for maximum production of ethanol from bagasse.