Monday, July 27, 2009
P22

Biobutanol Production from Ceylon Moss Sacchrified Liquid (CMSL) Using Solventogenic Clostridia

Younga Kim1, Tae Young Kim1, Gyung Soo Kim2, Myung-Kyo Shin2, Yong Jin Kim2, Jeong-Jun Yoon2, and In Seop Chang1. (1) Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju, South Korea, (2) Green Ocean Technology Center, Green Chemistry & Manufacturing System Division, Korea Institute of Industrial Technology (KITECH), 35-3 Hongcheon-ri, Ipjangmyeon, Chungnam, 330-825, Korea, Cheonan, South Korea

Many attempts were made to produce biofuels to substitute petro-based energy. Among them, bioethanol production was thought to be the most promising technology. However, recently, it has been known that butanol has several advantages over ethanol: higher energy density, less hygroscopic, etc. Due to those properties, butanol is considered as suitable biofuel for the real application without any modification of infrastructures that already installed. However, there is a huge challenge to produce biobutanol: the source of biomass – it has been shared with bioethanol. Ceylon Moss is a marine biomass which is easily obtainable near coasts of several areas (Korea, Japan, India and the Atlantic). There is low lignin content in Ceylon Moss; therefore, saccharification step was relatively easier than other lignin containing biomass. Herein, Ceylon Moss Saccharified Liquid (CMSL) was used to produce butanol by solventogenic clostridia such as Clostridium acetobutyricum, Cl. beijerlinkii, Cl tetanomorphum and Cl. aurantobutyricum. All tested clostridia were successfully cultivated on the Reinforced Clostridia Medium which was modified by substitution of CMSL (50%) instead of glucose and starch. Galactose (20 g/L) as main carbohydrate component in CMSL was used as carbon and energy source. Like typical two-phase ABE fermentation, pH change made it to shift solvent products; n-butanol was produced 2-5 mM with small amount of ethanol (0.3 mM) and acetone (3-4 mM) when batch culture was conducted in 72 hrs. Our results implied that CMSL can be good candidate of raw biomass for butanol production.
(Supported by Ministry of Knowledge Economy in Korea, 2008-N-BI08-P-01)

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