Monday, July 27, 2009
P4

Production of carotenoid-enriched whole stillage and wheat bran by fermentation using Phaffia rhodozyma and Sporobolomyces roseus

Nanjunda Swamy Ananda and Praveen Vadlani. Grain Science and Industry, Kansas State University, 1980, Kimball Ave, BIVAP Building, Manhattan, KS 66506

Whole stillage in the form of Dried Distiller’s grain with Solubles (DDGS) and wheat bran are routinely used as animal feed. Whole stillage is a by-product of corn-based ethanol industry, while wheat bran is a by-product of wheat milling. Carotenoids are added separately to animal feed to enhance meat color and quality, vitamin-A levels in milk and meat, and reproductive efficiency. To overcome the external addition of expensive carotenoids, this study aimed to produce carotenoid-enriched whole stillage and wheat bran by fermentation of yeasts namely, Phaffia rhodozyma and Sporobolomyces roseus. Carotenoids, astaxanthin and β-carotene are produced by P. rhodozyma, while S. roseus produces only β-carotene. Submerged fermentation was carried out at 20°C for 12 days and analyzed for carotenoid production by HPLC. Both, astaxanthin and β-carotene were successfully produced on whole stillage: P. rhodozyma produced 90µg of astaxanthin and 100µg of β-carotene, and S. roseus produced 750µg of β-carotene per gram of freeze dried whole stillage. Similarly, on wheat bran, P. rhodozyma produced 40µg of astaxanthin and 20µg of β-carotene, and S. roseus produced 15µg of β-carotene per gram of wheat bran. Our results show that value addition of whole stillage and wheat bran can be brought about by fermentation. Carotenoid-enriched whole stillage and wheat bran are cost effective and can be used to develop feed blends. Results from wheat bran solid state fermentation will be discussed and yield compared with that from submerged fermentation.

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