Monday, July 27, 2009 - 8:30 AM
S148

Disposable, orbitally shaken bioreactor

Tibor Anderlei1, Florian Wurm2, Stephanie Tissot3, Xiaowei Zhang4, and Cedric Bürki2. (1) Science and Business Development, Adolf Kuhner AG, Dinkelbergstr. 1, Birsfelden (Basel), 4127, Switzerland, (2) ExcellGene SA, Rte de l'Ile au Bois 1A, Monthey, 1870, Switzerland, (3) Lbtc, Swiss Federal Institute of Technology Lausanne, CH C1 392, Lausanne, 1015, Switzerland, (4) Lbtc, EPFL Lausanne, Batiment, Lausanne, 1005, Switzerland

A partnership of ExcellGene with Kühner AG was formed several years ago in order to develop a novel bioreactor principle that proves to be a simple and efficient bioreactor system. The cylindrical bioreactors, ranging in scale of operation from 1 mL to 150 Liter (volume of cell culture) are mounted on a circular moving platform. The mechanism of orbital shaking allows for efficient mixing of suspension cultures, while oxygenating cells through the head-space. At the 100 Liter scale the cylindrical vessel contains a disposable, sterile bag with appropriate connection tubes for seeding, feeding, gas supply and harvesting of the culture. Compared to classical stirred tank bioreactors, orbitally shaken bioreactors are much simpler and less costly to operate.

Data obtained have shown that oxygen, the most important nutrient in animal cell culture, can be transferred to cells efficiently in the shaken bioreactor system. Cell culture data obtained have shown that growth and productivity from these reactors, now shown up to a scale of 100 Liters (or even 1000L), are equivalent and frequently better than those of fully instrumented non-disposable, glass or stainless steel stirred bioreactors.



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