Thursday, August 14, 2008 - 5:00 PM
S164

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars

Stephen R. Hughes1, Jeremy Javers2, Joshua LaBeer3, Tauseef Butt4, Ronald E. Hector5, Bruce S. Dien6, Badal C. Saha7, Kenneth M. Bischoff8, Scott Kohl2, Michael A. Cotta9, and Joseph O. Rich10. (1) Bioproducts and Biocatalysis Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural, 1815 North University Street, Peoria, IL 61604, (2) ICM, Inc, 310 North First Street, P.O. Box 397, Colwich, KS 67030, (3) Harvard Institute of Proteomics, 320 Charles St, Cambridge, ME 02141-2023, (4) LifeSensors Inc., 271 Great Valley Parkway, Suite 100, Malvern, PA 19355, (5) Fermentation Biotechnology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL 61604, (6) National Center for Agricultural Utilization Research, USDA-ARS, Midwest Area, 1815 N. University Street, Peoria, IL 61604, (7) National Center for Agricultural Utilization Research, USDA, ARS, Midwest Area, 1815 N. University Street, Peoria, IL 61604, (8) National Center for Agricultural Utilization Research, USDA - Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, (9) Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, 1815 N. University Street, Peoria, IL 61604, (10) Bioproducts and Biocatalysis Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N. University Street, Peoria, IL 61604

Advanced automated high-throughput mass transformation of yeast full-genome libraries into Saccharomyces cerevisiae and screening for growth on xylose produced a yeast strain that is capable of fully utilizing pentose as well as hexose sugars anaerobically.  This is the first yeast strain capable of strictly anaerobic growth on xylose and glucose simultaneously.  Further study of this C5/C6 fermenting strain indicates optimum utilization of sugars from an acid hydrolysate of lignocellulosic feedstock.  The paradigm for use of the new strain in industrial lignocellulosic ethanol production using acid pretreatment will be discussed, including the regulatory considerations associated with this genetically engineered Saccharomyces cerevisiae strain.

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