Monday, August 11, 2008 - 11:00 AM
S25
Production of higher alcohols as biofuels
Shota Atsumi and James C. Liao. Chemical and Biomolecular Engineering, University of Californa, Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095
Global energy and environmental problems have stimulated increased efforts in synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared to their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a renewable carbon source, glucose. This strategy leverages the host’s highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high yield, high specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.
See more of Strain improvement and metaboic engineering for chemicals and fuels
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See more of The Annual Meeting and Exhibition 2008 (August 10 - 14, 2008)
See more of Invited Oral Papers
See more of The Annual Meeting and Exhibition 2008 (August 10 - 14, 2008)