Four strains of Bacillus amyloliquefaciens (designated 1D3, 1BC, 1BE, and 1BA) have been studied as biocontrol agents antagonizing plant pathogenic fungi. These biocontrol strains have not been previously assayed for xylanase and carboxymethylcellulase (CMCase) activity. In this study, plate assays were conducted with two CMC-containing media and one xylan-containing medium, with incubation at both 27 Celsius and 45 Celsius. Medium CMC-1 had a high C/N ratio and yeast extract as nitrogen source. Medium CMC-2 had a much lower C/N ratio and ammonium sulfate as nitrogen source. Ratios of zone of clearance diameter to colony diameter were compared. On CMC-1, the ratio for strain 1BA was about the same at both temperatures, but for the other three strains, ratios were about twice as large at the higher temperature, with values up to 4.7. On CMC-2, the ratio value of 4.6 for strain 1BA was about 1.4 times as large at the higher temperature; while ratios for the other three strains were approximately the same at both temperatures. At 27 Celsius, ratios for strains 1BC and 1BE were twice as large on CMC-2 as on CMC-1. For xylanase activity at 27 Celsius, strain 1D3 had the lowest ratio of 2.8, while all other strains had ratios near or above 4. At 45 Celsius, no zones of clearance were present for any strain besides that below the colonies.