Sunday, August 10, 2008
P135

Effect of Biosurfactant on Cellulase and α - Amylase Production by Phanerochaete Chrysosporium Under Solid Substrate Fermentation (SSF)

Chinonso Ekwueme1, Chukwuemeka C. Nwankwo1, Briggs. D Harry1, Ufot C. Essien1, and Nnanna C. Okrika2. (1) Medical Biochemistry and INTERNAL MEDICINE, MADONNA UNIVERSITY TEACHING HOSPITAL,, ELELE,, Rivers State, Nigeria, (2) Applied Biochemistry, UNIVERSITY OF CALABAR, CALABAR, Cross RIVER State, Nigeria

The effects of biosurfactant rhamnolipid were tested on the production of cellulase and α- amylase from Phanerochaete chrysosporium in solid substrate fermentation (SSF). The effects of rhamnolipid were also compared with those of chemical surfactants Tween 20, Tween 80 and SDS. The results showed that rhamnolipid at different concentrations enhanced activities of Avicelase, CMCase and cellobiase to different extents. During the peak enzyme production phase, rhamnolipid and Tween 80 increased cellulase activities by 28–59%. However, in the later fermentation phase, the activities of Avicelase and cellobiase in the presence of rhamnolipid were 100.5% and 164.8% higher than those of the control, respectively. Both rhamnolipid and Tween 80 had the pronounced stimulatory effects on α- amylase during the peak enzyme production than Tween 20 and SDS. Rhamnolipid at 0.029% (w/w) gave the highest α- amylase activity, which was 119.6% higher than the control. In the whole, the stimulatory effects of rhamnolipid were superior to those of Tween 20, Tween 80 and SDS.

 



Web Page: www.surfactants.com

See more of Poster Session 1
See more of Posters

See more of The Annual Meeting and Exhibition 2008 (August 10 - 14, 2008)