Sunday, August 10, 2008
P27
Isolation and selection of thermotolerant fungal from hot compost, for the delignification of sugarcane bagasse
A. T. Cervantes - Rodriguez, K. N. Rivera - Hernandez, and S. R. Trejo - Estrada. Biotechnology, Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, CIBA-IPN Tlaxcala, Carretera Estatal Santa Ines Tecuexcomac - Tepetitla Km. 1.5, Exhacienda San Juan Molino., Tepetitla, Mexico
Filamentous fungi produce a number of extracellular oxidative enzymes including laccase, lignin peroxidase, and manganese peroxidase, involved in the breakdown of lignin, the plant structural material lignin. Enzymes from thermophylic organisms are more thermoestable, increasingly resistant to proteolysis, and more resistant to mechanical denaturation than those from mesophilic strains. In the present study we report the isolation of 29 thermophilic fungal strains able to produce laccase, lignin peroxidase, and manganese peroxidase. The strains were isolated from compost samples taken during the the thermophilic phase. The composting mixture is made of sugarcane bagasse and filter mud. Highly diverse microbial communities associated with filter mud originate in tropical rainforests from Central Veracruz, in the Gulf of Mexico. Selective media for basidiomycotina allowed for the selection of brown-rot and white-rot fungal strains which readily colonize non-steamed raw bagasse at high temperature (>60 °C). Enzyme analysis shows the production of at least one of the enzymes involved in delignification at high temperatures. Lignolytic enzymes have been also analyzed from fermentation broths in liquid culture.
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