Monday, August 11, 2008
P78

Medium Development for Riboflavin Production using Experimental Design

Gaby Tiemi Suzuki and Gabriela Alves Macedo. Food Science Department, University of Campinas - Unicamp, R Monteiro Lobato, 80 PO Box 6121, Barão Geraldo 13083862, Campinas-SP, Brazil

Having to choose the best medium so that a microorganism produces its metabolites is laborious and time-consuming. Statistical design is a tool in which planned testing occurs so as to have a very scientific approach. Another advantage would be the variation of the factors simultaneously, resulting in an improved analytical methodology, reducing the number of experiments or repetitions. Therefore, in order to optimize the production of a metabolite, it seems to be a very useful tool. Riboflavin is a yellowish-green fluorescent pigment produced by plants and microorganisms, which in recent years has been found to be effective in the treatment of migraine, malaria and Parkinson's disease. Riboflavin is one of the fewest vitamins B biotechnologically produced. We previously isolated and screened riboflavin-producer yeast in our laboratory from soil sample collected from sugarcane Plant. It was tested in a hyper-osmotic medium in a small-scale fermentation process for riboflavin production. Its production may be stimulated by many different compounds including industrial residues. Hence, after studying the influence of different carbon and nitrogen sources, a 25 design was used to analyze the effect of the medium components such as sucrose, flaxseed oil and Prodex Lac@, resulting in a positive effect for sucrose. Sucrose’s concentration varied from 50g/L to 100g/L. Changing medium composition caused a 10-fold better production of the metabolite. Classical shaken fermentation vessel system was used. For riboflavin analysis, a spectrophotometric determination was done. Screening design as a medium performance evaluation ended up being very advantageous for riboflavin production by this yeast.

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