Monday, August 11, 2008 - 9:50 AM
S15
Production of Pyruvate by Metabolically Engineered Escherichia coli
Yihui Zhu, Mark A. Eiteman, and Elliot Altman. Cmbe, University of Georgia, Driftmier, Athens, GA 30602
Although several microorganisms accumulate pyruvate by the conversion of glucose and other renewable resources, Escherichia coli has many advantages including rapid growth, simple nutritional requirements and the ease of genetic manipulation. A series of steady-state (chemostat) experiments were conducted to evaluate the ability of metabolically engineered E. coli strains (pflB aceEF poxB pps ldhA) to produce pyruvate under several different nutrient-limited conditions. A goal for pyruvate formation is high glycolytic flux (relative to biomass formation). The greatest specific glucose consumption rate and hence pyruvate formation rate was found under conditions of acetate-limited growth. Pyruvate productivity was further increased by introducing an ATP synthase knockout (atpFH), an arcA knockout and by introducing heterologous NADH oxidase. These chemostat studies were successfully adapted to a well-controlled fed-batch process operated at a low specific growth rate of 0.15 h-1. Under these fed-batch conditions, pyruvate concentrations above 80 g/L and productivities exceeding 2 g/L·h were achieved.
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See more of The Annual Meeting and Exhibition 2008 (August 10 - 14, 2008)