Monday, August 11, 2008
P20

DiaSync™ TFF Systems Decrease Environmental Impact of Downstream Processes

James A. Kacmar, Mike Eggers, and Henry B. Kopf. NCSRT, Inc., 1000 Goodworth Dr., Apex, NC 27502

With the increasing productivity of fermentation processes, the economical and environmentally safe purification of  fermention products has become a bottleneck in the manufacture of industrial biomolecules.  Typically, large quantities of buffer must be made at considerable expense to diafilter the product away from the cells and cell debris.  After the product is isolated, this buffer must be disposed of or treated.  The DiaSync™ tangential flow filtration (TFF) production skid combines the product harvest and product concentration steps into one system.  Using SmartFlow™ membrane technology, the product is harvested using a microfiltration (MF) membrane in the first step and concentrated using an ultrafiltration (UF) or nanofiltration (NF) membrane in the second step.  The permeate from the UF or NF step is then recycled back for use as a diafiltration buffer for the MF step.  This recycling of buffer greatly reduces the cost of buffer preparation, Quality control, and the cost of buffer disposal.  Here, data is shown for the use of the DiaSync system for multiple industrially relevant fermentation platforms.  Recoveries of over 85% and the elimination of thousands of liters of buffer to be disposed of are typical for the DiaSync system, which permits one to Go Green Downstream.

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