Monday, August 11, 2008
P54
Efficient Production of Lactic Acid by Metabolically Engineered Saccharomyces cerevisiae
Saccharomyces cerevisiae is a robust industrial production platform for various therapeutic protein, high added-value chemicals, and commodities. However, in order to maximize the desired product yield, it is necessary to redirect carbon fluxes away from alcoholic fermentation towards the desired product and to minimize other by-products.
In the case of lactic acid mass production, in order to develop the strain which can produce it efficiently, the coding regions of pyruvate decarboxylase 1 (PDC1) and PDC5 were substituted for that of the L-lactate dehydrogenase (LDH), and glycerol-3-phosphate dehydrogenase 1 (GPD1) was disrupted. This recombinant produced L-lactate in high yield, however, led to decrease in the cell growth and the fermentation rate.
To solve these problems, mutation breeding was studied.
As a result, the cell growth and the fermentation rate of mutants fully recovered. The fermentation yield was also improved, especially they produced L-lactate in high yield even in nutrient-poor medium. In addition, other fermentation characteristics will be presented.
In the case of lactic acid mass production, in order to develop the strain which can produce it efficiently, the coding regions of pyruvate decarboxylase 1 (PDC1) and PDC5 were substituted for that of the L-lactate dehydrogenase (LDH), and glycerol-3-phosphate dehydrogenase 1 (GPD1) was disrupted. This recombinant produced L-lactate in high yield, however, led to decrease in the cell growth and the fermentation rate.
To solve these problems, mutation breeding was studied.
As a result, the cell growth and the fermentation rate of mutants fully recovered. The fermentation yield was also improved, especially they produced L-lactate in high yield even in nutrient-poor medium. In addition, other fermentation characteristics will be presented.
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