Monday, August 11, 2008
P8
Optimization of capsular polysaccharide production from Streptococcus pneumoniae
Sheng-De Jin1, Young-Min Kim2, Hee-Kyoung Kang3, and Doman Kim2. (1) School of Biological Sciences and Technology, The second stage of BK21, Korea Ministry of Education, Chonnam National University, 300 Yongbong-dong, Gwangju, 500-757, South Korea, (2) School of Biological Sciences and Technology, The second stage of BK21, Korea Ministry of Education, The Research Institute for, Chonnam National University, 300 Yongbong-dong, Gwangju, 500-757, South Korea, (3) The Research Institute for Catalysis, Chonnam National University, 300 Yongbong-dong, Gwangju, 500-757, South Korea
The production of capsular polysaccharide (CPS) from Streptococcus pneumoniae Type 3 in defined medium was studied. First the medium components were selected in accordance with the Plackett-Burman design and then further optimized by response surface methodological approach. Three significant variables (glucose, yeast extract, and KH2PO4) were found to be important factors. Through the central composite design (CCD), the optimal value for each factor was 0.9% glucose, 6% industrial yeast extract, and 0.2% KH2PO4 in the aspect of process simplicity and production cost. By using optimal composition, CPS production was increased about 2.5-fold compared to that of the BHI medium supplemented with 1% glucose. The CPS purification procedure was also optimized for cell breakage, centrifuge, EtOH precipitation and following GPC with Sephacryl S-300HR.
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