Monday, August 11, 2008
P116
The group 2 sigma factor SigB positively regulates glucose metabolism under oxygen-deprived conditions in Corynebacterium glutamicum
Shigeki Ehira, Haruhiko Teramoto, Masayuki Inui, and Hideaki Yukawa. Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizugawa, Kyoto, Japan
Corynebacterium glutamicum is a non-pathogenic, high-GC, gram-positive bacterium used for the industrial production of several amino acids, such as L-glutamate and L-lysine. We have developed processes for the production of organic acids and ethanol from sugars at high productivity using growth-arrested C. glutamicum strain R cells under oxygen-deprived conditions. The increased rate of glucose consumption under the conditions accompanies up-regulation of genes encoding several key enzymes of the glycolytic and organic acid production pathways. Regulatory mechanisms of gene expression under oxygen-deprived conditions still remain to be unraveled.
The sigB gene of C. glutamicum encodes a group 2 sigma factor of RNA polymerase, which is up-regulated under oxygen-deprived conditions. Using DNA microarray and quantitative RT-PCR analyses, we found that the sigB disruption reduced transcript levels of genes involved in conversion from glucose to organic acids accompanied with retardation of glucose consumption under oxygen-deprived conditions. These results indicate that SigB positively regulates glucose metabolism genes and enhances glucose consumption under oxygen-deprived conditions. This work was partially supported by a grant from the New Energy and Industrial Technology Development Organization (NEDO).
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