Wednesday, August 13, 2008 - 10:30 AM
S109

Detection of Biological Agents/Pathogens in Food

Charles C. Young, Alex J. Proescher, Emily E. Seay, and Sarah L. Grady. National Security and Technology, The Johns Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins Road, Laurel, MD 20729

The intentional contamination of food supplies with biological agents represents a significant bioterrorism threat with a number of unique challenges for future biological sensing systems and the technologies upon which they will be based.  These include, but are not limited to matrix effects, the current reliance on enrichment methods, issues surrounding natural “background contamination/gene transfer, and appropriate target selection.  It is envisioned that next generation biosensors will generate near real time results using multiple sensing modalities to yield orthogonal confirmation of biowarfare agents in matrices without a requirement for culture.  These sensors are also expected to integrate sample preparation, amplification, agent detection, and data fusion/analysis into a single, small footprint for use by relatively untrained individuals.  While significant investment and effort has been made over the past 5 years developing new detection methods/systems, development of integrated sample preparation and data fusion algorithms have lagged behind.  This presentation will focus on sample preparation from food matrices that have traditionally proven difficult for automated biodetection systems and the interface between the need to test statistically significant sample volumes and microfluidic systems.