Monday, August 11, 2008 - 3:00 PM
S41
Effective 1,4-dihydroxy-2-naphthoic acid production by Propionibacterium freudenreichii ET-3
Keisuke Furuichi and Shinichi Ohki. Production Technology and Engineering Department, Meiji Dairies Corporation, 540 Naruda, Odawara, Kanagawa, 250-0862, Japan
Propionibacteria, particularly Propionibacterium freudenreichii ET-3, has been found to produce a novel bifidogenic growth stimulator (BGS). The chemical substance that confers the major BGS activity in ET-3 culture was identified as 1,4-dihydroxy-2-naphthoic acid (DHNA), which is a precursor of menaquinone (MK). It was demonstrated that taking tablets containing ET-3 culture significantly increases the ratio of bifidobacteria population in the human intestine. Moreover, taking tablets containing ET-3 culture decreases the concentrations of fecal indole and skatole, which are putrefactive metabolites produced by intestinal bacteria, and increases the stool frequency. Therefore, tablets or food containing ET-3 culture can be considered to benefit human health.
In order to use ET-3 culture containing DHNA foods, it is necessary to produce it at a low cost. Our aim in this study was to develop a culture method to increase DHNA concentration in the ET-3 culture. Under aerobic conditions, ET-3 consumed propionate, which is the major inhibitory factor for DHNA production, and produced DHNA steadily and continuously. Lactose-limiting fed batch culture inhibited MK production and thus increased DHNA concentration, because almost half of the DHNA produced by ET-3 results in MK production under normal anaerobic culture conditions. Finally, we conducted a combined aerobic culture and lactose-limiting fed batch culture to maximize the benefits of the two above cultures. The DHNA concentration in this combined culture was 0.52 mM, which is 3.7-fold that in a normal anaerobic culture.
In order to use ET-3 culture containing DHNA foods, it is necessary to produce it at a low cost. Our aim in this study was to develop a culture method to increase DHNA concentration in the ET-3 culture. Under aerobic conditions, ET-3 consumed propionate, which is the major inhibitory factor for DHNA production, and produced DHNA steadily and continuously. Lactose-limiting fed batch culture inhibited MK production and thus increased DHNA concentration, because almost half of the DHNA produced by ET-3 results in MK production under normal anaerobic culture conditions. Finally, we conducted a combined aerobic culture and lactose-limiting fed batch culture to maximize the benefits of the two above cultures. The DHNA concentration in this combined culture was 0.52 mM, which is 3.7-fold that in a normal anaerobic culture.
See more of Advances in Fermentation Technology
See more of Invited Oral Papers
See more of The Annual Meeting and Exhibition 2008 (August 10 - 14, 2008)
See more of Invited Oral Papers
See more of The Annual Meeting and Exhibition 2008 (August 10 - 14, 2008)