Monday, August 11, 2008
P4
Isolation and Characterization of Aspergillus Niger Capable of Utilizing ĮLpha-Methyl-D-Glycoside, a Synthetic Analogue of Maltose, as Substrate in the Production of Amylase
Uche Ernest Nnamani and Okechukwu Michael Nwafor. Applied Biology, Enugu State University of Science and Technology, AGBANI, ENUGU, Enugu State, Nigeria
A strain of Aspergillus niger isolated from raw native starch was able to produce biomass and high amylase activities in media containing alpha-methyl-d-glucoside (αMG), a synthetic analogue of maltose, as the only carbon source. αMG was a more effective inducer than starch and maltose at the same concentration: αMG cultures produced about 3 times more α-amylase and glucoamylase activity than starch cultures. Maximum production of alpha-amylase (70 U/mg) and glucoamylase (145 U/mg) was obtained in 9–12days alpha-methyl-d-glucoside cultures.
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