Monday, August 11, 2008
P48
Evaluation of the Optimum Fermentation Conditions for the Enhanced Production of Biosurfactants from an Actinomycete by the Application of Response Surface Modelling
Chukwudi Ogbonnaya Onwosi and Fredrick J.C. Odibo. Biotechnology Research Center, NNAMDI AZIKIWE UNIVERSITY, P.M.B. 5025, Awka, Anambra State, Nigeria
The production of a glycolipid biosurfactants by an actinomycete isolated from compost was investigated in shake flask fermentation. Response surface methodology (RSM) was applied to optimize the fermentation process conditions (pH, agitation rates, temperature and aeration) on glycolipid production. A 24 full factorial central composite design followed by multi-stage Monte-Carlo optimization was used for experimental design and analysis of the results. This approach reduces the number of experiments while at the same time obtaining the mutual interactions between the variables required for achieving optimal experimental conditions. Thus, the effects of the mutual interactions between these parameters were well monitored to enhance the process conditions for the maximization of biosurfactants production. The optimal fermentation conditions for the enhanced production of glycolipid biosurfactants were temperature 36.5oC, agitation of 180rpm, pH of 7.24 and aeration of 0.80vvm. The actual experimental results were in agreement with the prediction by the RSM
Web Page: www.biosurfactants.org
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