Monday, August 11, 2008
P46
Optimization of the Culture Media for the Production of Glycolipids from an Actinomycete isolated from Compost Using Response Surface Methodology
Chukwudi Ogbonnaya Onwosi and Fredrick J.C. Odibo. Biotechnology Research Center, NNAMDI AZIKIWE UNIVERSITY, P.M.B. 5025, Awka, Anambra State, Nigeria
Actinomycetes produced surface active glycolipids when grown on mineral medium with carbon sourse as sucrose. This work presents an investigation on the optimization of the fermented culture medium for maximization of glycolipids production by an actinomycete isolated from compost using Response Surface Modeling (RSM). The nitrogen source (NH4)2SO4, carbon source (sucrose), phosphates (KH2PO4/K2HPO4 1:2) and the mineral salt (Fe2+) were the culture media components optimized. A 24 full factorial central composite experimental design and response surface methodology was used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the four parameters (sucrose, (NH4)2SO4, (KH2PO4/K2HPO4 1:2) and FeSO4.7H2O) on the production of biosurfactants. The maximum concentration of glycolipid, 20.9gL-1, was recorded in medium that contained, 40gL-1 sucrose, 3.0 gL-1 (NH4)2SO4, 1.2gL-1 phosphates and 0.2gL-1 FeSO4.7H2O. These results show optimum values for the tested variables.
Web Page: www.biosurfactants.org
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