Sunday, August 10, 2008
P7
Microbial production of glyceric acid from glycerol
H. Habe, D. Kitamoto, and K. Sakaki. Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan
Glycerol is produced as a by-product of the oleochemical and biodiesel industries. Since glycerol production is expected to expand further with increasing the use of biodiesel, it will lead to much greater availability and lower price of glycerol. Efficient treatment of raw glycerol is thought to be one of key factors for biodiesel manufacturing process. Conversion of glycerol with bioprocess has been extensively studied and several possible products such as 1,3-propandiol (1,3-PD), ethanol, acetic acid, 2,3-butanediol, succinic acid, lactic acid, formic acid, propionic acid, hydrogen gas, and 3-hydroxypropionaldehyde have been reported. However, there is little knowledge about the microbial production of glyceric acid from glycerol. In this study, we screened several bacterial strains belonging to the family of acetic acid bacteria (Acetobacteriaceae) converting glycerol to glyceric acid. By HPLC analysis, we found that most strains belonging to the genera Acetobacter and Gluconacetobacter were able to convert glycerol to glyceric acid. Among them, Acetobacter tropicalis NBRC16470 produced 25 g/L of D-glyceric acid at 0.5L fermentation scale when 15%(v/v) glycerol was supplied.
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