Sunday, August 10, 2008
P45

Potential application of thermostable xylanase from Bacillus pumilus in pretreatment of cotton and micropoly fabrics

Jitender Sharma1, Bindu Battan2, and Saurabh S Dhiman2. (1) Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India, (2) Biotechnology, Kurukshetra University, Kurukshetra, 136119, India

Desizing of cotton and micropoly fabrics was done using alkalostable and thermostable xylanase of Bacillus pumilus ASH. Micropoly fabric showed better desizing than cotton under same conditions. Violet scale readings from the TEGEWA test after enzymatic desizing for 90 min at pH 7.0 and at 60ºC showed the readings falling in the range of 4-5 indicating good desizing efficiency. During bioscouring the weight loss values and liberation of reducing sugars were highest when EDTA was used along with xylanase. The weight loss value of 1.5% was observed for cotton fabric after 1 h in case of agitated system at pH 7.0 and at an optimal enzyme dosage of 5 IU/g of the dry fabric. The weight loss values and the liberation of reducing sugars were higher in case of cotton fabrics.  Wetting time of fabrics was lowered significantly after 60 min of bioscouring using xylanase. Increase in temperature or concentration of surfactant led to further reduction in the wetting time. The bioscouring of fabrics with xylanase resulted in enhancement of various physical properties of the fabrics viz. whiteness, tensile strength and tearness by 0.9%, 1.12% and 1.1% respectively over the conventionally alkaline scoured fabrics indicating the efficacy of xylanase during the scouring process.

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