Filamentous fungi are the major practical source of cellulases and hemicellulases for industrial applications. Extensive work has been dedicated to understand the molecular mechanisms of enzymatic cellulose and arabinoxylan degradation. Chrysosporium lucknowense (C1) strains exhibit high levels of protein production and secretion as well as low viscosity. The nonfilamentous growth morphology of the strains motivated the development of a gene expression system in this fungal organism. This expression platform enables us to identify and characterize multiple carbohydrases and their hydrolytic patterns to select those which exhibit high catalytic efficiency in the saccharification of numerous types of lignocellulosic materials.