This optimization strategy was successfully utilized to increase protein production of a CHO line producing IgG1.  The cell line was first screened through a library of chemically defined media in order to identify a new base medium.  Key components in the selected formulation were further optimized to meet the specific nutritional requirements of the cell line.  Utilizing the optimized base medium, the cells were screened against a diverse panel of animal free peptones and an individual peptone was identified.  Several rounds of process optimization were then performed to identify the optimal production parameters.  Using the optimized media and process identified in this study, a 7-fold increase in protein production was achieved in less than a year.