Wednesday, August 1, 2007 - 9:30 AM
S139
Advanced approach using genome information to generate amino acid producers
Masato Ikeda, Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, Nagano, 399-4598, Japan
Based on the fruits of Corynebacterium glutamicum genomics, we have recently developed a novel approach that employs genome information to systematically create a minimally mutated producer. The methodology presented so far by us is identifying useful mutations from the genome of a classically derived producer and subsequent assembling them in a wild-type strain. By applying this methodology to L-lysine fermentation, we have demonstrated that the approach leads to generation of an efficient and tough producer as well as better understanding of production mechanisms.
With accumulated knowledge on mutations relevant to production, it becomes possible to combine positive mutations derived from different producers in a single wild-type background. In addition, optimization of key mutations through base substitutions remains to be attempted. Here we describe such advanced approach, as well as the essence of our methodology for strain reconstruction, by using L-lysine and L-arginine fermentations as models.
With accumulated knowledge on mutations relevant to production, it becomes possible to combine positive mutations derived from different producers in a single wild-type background. In addition, optimization of key mutations through base substitutions remains to be attempted. Here we describe such advanced approach, as well as the essence of our methodology for strain reconstruction, by using L-lysine and L-arginine fermentations as models.