Sunday, July 29, 2007
P37

A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products

Siqing Liu1, Kelly A. Skinner-Nemec2, and Timothy D. Leathers1. (1) USDA-ARS-NCAUR, 1815 N University, Peoria, IL 61604, (2) Argonne National Laboratory,, 9700 S. Cass Avenue,, Argonne, IL 60439

Lactobacillus buchneri strain NRRL B-30929 was isolated from a fuel ethanol production facility.  This heterofermentative, facultative anaerobe can utilize xylose as a sole carbon source and tolerates up to 12% ethanol.  Carbohydrate utilization (API, Biomerieux) and Phenotype Microarrays™ (PM, Biolog) analyses indicated that the strain is able to metabolize a broad spectrum of carbon sources, including various monosaccharides (C5 & C6), disaccharides, and oligosaccharides.  More substrates were used under anaerobic than aerobic conditions.  In pH-controlled bioreactors, NRRL B-30929 fermented glucose alone (10%, pH 4.0) to lactate (240 mM), ethanol, (232 mM) and acetate (57 mM).  This strain also utilized high concentrations of xylose and glucose simultaneously (12 % each, pH 6.0) to produce lactate, acetate and ethanol.  Another interesting product recovered in the fermentation broth is a 3.5 kDa peptide which was identified as buchnericin.  The simultaneous production of lactate, ethanol, and buchnericin from lignocellulosic biomass has potential application in bio-based refinery platforms.

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