Sunday, July 29, 2007
P107

iAF1260: A genome-scale metabolic model of E. coli as a platform for metabolic engineering

Adam M. Feist, Kirstin Tang, Markus J. Herrgård, and Bernhard Ø. Palsson. Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093

The use of systems biology methods in metabolic engineering applications is on the rise. Aiding these efforts are models that allow for the analysis of cellular processes on the genomic-scale and the integration of high-throughput data sets. Genome-scale metabolic models specifically are important in metabolic engineering applications due their ability to predict the effects of specific genetic manipulations on substrate uptake or desired byproduct secretion. Here, we present iAF1260, a genome-scale metabolic reconstruction of E. coli which contains 1260 ORFs from the latest genome annotation and over 2000 transport and intracellular reactions as an analysis platform for metabolic engineering. We characterize the reconstruction, outline its current usage and describe further areas for improvement and implementation in product-driven applications.

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