Sunday, July 29, 2007
P71
Proteomic analysis of Pseudomonas aeruginosa to E,E-farnesol, a quorum-sensing compound of Candida albicans using 2D DIGEŽ technology
Shelby L. Jones and George E. Pierce. Biology, Georgia State University, 24 Peachtree Center Ave., Atlanta, GA 30303
Nosocomial infections associated with implanted medical- devices are on the rise due to a growing immunocompromised patient population. The organisms of interest here are Pseudomonas aeruginosa and Candida albicans. These organisms are classified as opportunistic pathogens and are highly associated with medical- device infections. Pseudomonas aeruginosa is a motile gram-negative bacterium that is able to suppress the growth of Candida albicans. Quourm sensing mimicry and biofilm formation on the hyphal surface of C. albicans by P. aeruginosa aid suppression. C. albicans is a dimorphic fungus capable of quorum sensing with E,E-farnesol and is the focus of this work. The goal of this project is to determine changes in protein expression when P. aeruginosa is exposed to E,E,-farnesol using 2D DIGE®. This work summarizes the effect of growth phase and concentration of E,E-farnesol on P. aeruginosa PAO1 and GSU3. Preliminary results reveal a general response of P. aeruginosa to C. albicans as changes in relevant metabolic nodes that result in phenazine production and the induction of virulence factors that lead to the killing of C. albicans. The overall goal of this study was to generate a profile of protein expression where a variety of conditions to further characterize the response could be easily assayed.
See more of Poster Session 1
See more of Posters
See more of The Annual Meeting and Exhibition 2007 (July 29 - August 2, 2007)
See more of Posters
See more of The Annual Meeting and Exhibition 2007 (July 29 - August 2, 2007)