Sunday, July 29, 2007
P31
A complete sequencing and thermostable gene products of the genome of the hyperthermophilic archaeon Thermococcus onnurineus NA1
Hyun Sook Lee, Sung Gyun Kang, Yun Jae Kim, Jae Kyu Lim, Seung Seob Bae, Jeong Ho Jeon, Yona Cho, Sang-Jin Kim, and Jung-Hyun Lee. KORDI, Ansan PO Box 29, Seoul, 425-600, South Korea
Recently, we isolated a new hyperthermophilic strain from a deep-sea hydrothermal vent area in the PACMANUS field, and assigned as Thermococcus onnurineus based on polyphasic taxonomical approach. The genus Thermococcus is ubiquitously found in natural high-temperature environments, and may play a major role in the ecology and metabolic activity of microbial consortia within thermal ecosystems. To obtain information for the understanding living mechanisms of the metabolic activity as well as for the highly thermostable gene products, the complete genome sequences of this strain was determined, followed by an extensive annotation of open reading frames (ORFs). The entire length of the genome was 1,847,607-bp (52.3% G+C) with 2,030 predicted protein-encoding genes. The full ORFs of T. onnurineus NA1 were compared with those from the complete genomes of archaea, especially with those of thermococcales. The genomic scale phylogeny was also considered. To develop novel valuable thermostable enzymes from T. onnurineus NA1, the bioinformatic and biochemical approaches such as genome context analysis, gene cloning and enzyme characterization were carried out.
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