Sunday, July 29, 2007
P29
The development of enantioselective epoxide hydrolases from marine microorganisms
Sung Gyun Kang, Young-Ok Hwang, Jung-Hee Woo, Ji-Hyun Kang, and Sang-Jin Kim. Marine Biotechnology Centre, Korea Ocean Research & Development institute, Sa 2 dong 1270, Sangrok-gu,, Ansan, 426-744, South Korea
To develop epoxide hydrolases from marine microorganisms, genomic information of marine microorganisms has been analyzed using various bioinformatic tools. Three genes encoding putative EHases were identified by analyzing open reading frames (ORFs) of Erythrobacter litoralis HTCC2594. Despite low similarities to reported EHases, the phylogenetic analysis of the three genes showed that eeh1 was similar to microsomal EHase while eeh2 and eeh3 could be grouped with soluble EHases. In addition, genes encoding putative EHases were also identified by analyzing open reading frames (ORFs) of several marine microorganisms whose genome sequences are available in public. The putative epoxide hydrolases from marine microorganisms were cloned and expressed in E. coli. The recombinant EHases were purified by metal affinity chromatography and further characterized. The application to chiral resolution of racemic epoxide substrates by purified enzymes is under progress.
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