Tuesday, July 31, 2007 - 3:00 PM
S114A
Yeast and microbial fermentation in a stirred tank disposable bioreactor
Patricia Puma, Manufacturing, Xcellerex Inc., 170 Locke Drive, Marlborough, MA 01752
The Xcellerex disposable reactor (XDR™) was evaluated for its ability to support fermentation of a yeast cell line. Prior to this, a number of 200 L and 1000 L mammalian cell culture runs had been done in the XDR™. While the reactor design used for those runs was primarily targeted to the requirements of mammalian and insect cultures, the performance and capabilities of the reactor suggested that the XDR™ could also be suitable for lower density yeast and microbial applications, and that this single-use system could provide a rapid and relatively cost-effective method for scale-up for antibody production in these systems. The Saccharomyces cerevisiae process, run previously in a conventional stainless steel bioreactor at high density, was adapted to fermentation in the XDR™-200 stirred tank disposable bioreactor. Data are presented demonstrating that the current XDR™-200, with little to no modification, can successfully support low density yeast fermentation cultures.