Sunday, July 29, 2007
P25
Development of emulsion system for increased retinal production from beta–carotene by beta-carotene 15,15'-monooxygenase
Nam-Hee Kim, Yeong-Su Kim, Hye-Jung Kim, and Deok-Kun Oh. Department of Bioscience and biotechnology, Konkuk University, Seoul, South Korea
Retinal was produced from the cleavage of β-carotene by β-carotene 15,15’-monooxygenase. In the production, a classical technological problem rose as the enzyme worked in an aqueous phase and β-carotene as a substrate was insoluble in water. To increase the retinal production, the emulsion system was developed by optimizing kinds and concentrations of solvent and detergent, and substrate concentration. The emulsion system consisted of 100 mg/L β-carotene and 2.4% tween 80 in toluene. Using the emulsion system, about 45 mg/L retinal was obtained after 37 h with a productivity of 1.22 mg/L·h.
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